covid 19 Diagnosis test series

covid 19 Diagnosis test series

CDC has developed two laboratory tests that identify SARS-CoV-2, the virus that causes COVID-19. The first test for COVID-19 diagnosis that CDC distributed, released in February 2020, is the CDC 2019-Novel Coronavirus (2019-nCoV) Real-Time RT-PCR Diagnostic Panel, a test that accurately detects SARS-CoV-2 in respiratory specimens. CDC released a second assay in July 2020, the CDC Influenza SARS-CoV-2 (Flu SC2) Multiplex Assay, which permits public health laboratories to run three tests in a single reaction well. The Flu SC2 Multiplex is more efficient in its use of test reagents, allows higher throughput, and simultaneously gives accurate results about the presence of SARS-CoV-2, influenza A, and influenza B nucleic acid in a patient specimen. Symptoms for COVID-19 and the Flu can be similar, so testing for all three viruses at the same time will provide public health officials with information they need to help reduce the spread of these viruses in the community while conserving resources that are in short supply.

Please note: CDC will discontinue the CDC 2019-nCoV Real-time RT-PCR Diagnostic Panel at the end of 2021. For more details, please see the CDC 2019-nCoV Real-time RT-PCR Diagnostic Panel section below.

Introduction

Compared to 2002/2003 SARS-CoV and 2012–2014 MERS-CoV epidemics, COVID-19 coronavirus rapidly spread to other parts of the world (185 countries and territories, Last updated: March 21, 2020)().

In symptomatic patients, the clinical manifestations of the disease usually start after less than a week, consisting of fever (body temperature 37 to 38°C), cough, nasal congestion, and fatigue (). Pneumonia mostly occurs in the second or third week of a symptomatic infection (). Comparison of hematological parameters between mild and severe cases of COVID-19 showed significant differences in interleukin-6 (IL-6), D-Dimer, glucose (GLU), thrombin time (TT), fibrinogen (FIB) and C-reactive protein (CRP) (). Fan et al. analyzed the hematological indices of COVID‐19 infected patients between the intensive care unit (ICU) and non‐ICU patients. They showed lymphopenia and raised lactate dehydrogenase (LDH) were associated with higher rate of ICU admissions. Patients who were transferred to the ICU had a lower nadir lymphocyte count, nadir monocyte count and nadir hemoglobin, and higher peak Neutrophil (NEU) Count and peak LDH levels compared to patients who did not require ICU stay () . Many patients with MERS-CoV had liver function abnormalities with elevated alanine aminotransferase (ALT), aspartate aminotransferase (AST), and LDH (). Also laboratory data on SARS have shown that most patients had elevated CRP levels, lymphopenia, leukopenia, and elevated levels of aminotransferase, LDH and creatine kinase (). A series of recently published articles have reported the epidemiological and clinical characteristics of patients with COVID-19 disease, but data regarding the laboratory characteristics of infected individuals are limited (). This study aimed to evaluate the accuracy of laboratory parameters in predicting cases with positive RT-PCR for COVID-19.

covid 19 Diagnosis test series
    covid 19 Diagnosis test series

Methods

Study design and setting

This diagnostic accuracy study was conducted on suspected COVID-19 patients, who presented to Behpooyan Clinic Medical center in Tehran (Iran) from 22 February to 14 March, 2020. Patients were divided into two groups based on the results of real time reverse transcriptase‐polymerase chain reaction (RT-PCR) for COVID-19 and the accuracy of different laboratory parameters in predicting cases with positive RT-PCR was evaluated using area under the ROC curve (AUC). The study protocol was approved by the Ethics Committee of Shahid Beheshti University of Medical Sciences (ethical code: IR.SBMU.RETECH.REC.1399.010).

Participants

Outpatients with suspected COVID-19 having initial respiratory signs (including sore throat without shortness of breath), fever, cough, muscle ache, and headache were included ().

Data gathering

Pharyngeal swab samples were collected for COVID-19 test on presentation. Blood samples were collected from each participant and routine blood test including White blood cell count (WBC), Lymphocyte count (LYM), and Neutrophil count (NEU) were performed on the blood samples. Furthermore, blood biochemistry parameters such as Aspartate aminotransferase (AST), Alanine aminotransferase (ALT), Urea, C-reactive protein (CRP), as well as Albumin and lactate dehydrogenase (LDH) were assessed using HITACHI 7600-020 automated biochemistry analyzer.

Panbio™ COVID-19 Ag Rapid Test Device (Nasal)

41FK11 25 Tests/Kit
EUR 95
Description: The Coronavirus disease (COVID-19) is an infectious disease caused by a newly discovered coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The SARS-CoV-2 is a β -coronavirus, which is an enveloped non-segmented positive-sense RNA virus 2. It is spread by human-to-human transmission via droplets or direct contact, and infection has been estimated to have a mean incubation period of 6.4 days and a basic reproduction number of 2.24-3.58. Among patients with pneumonia caused by SARS-CoV-2, fever was the most common symptom, followed by cough3. The main IVD assays used for COVID-19 employ real-time reverse transcriptase-polymerase chain reaction (RT-PCR) that takes a few hours 4. The availability of a cost-effective, rapid point- of-care diagnostic test is critical to enable healthcare professionals to aid in the diagnosis of patients and prevent further spread of the virus5. Antigen tests will play a critical role in the fight against COVID-19

GENLISA™ SARS-CoV-2 (Covid-19) Surrogate Virus Neutralization Test (sVNT) ELISA

KBVH400 12 × 8 wells
EUR 3185

COVID-19 IgG & IgM antibody

GEN-50001-50tests 50 tests
EUR 324
Description: A rapid test for detection of antibodies (IgG and IgM) for 2019-nCoV, the novel Coronavirus from the Wuhan strain. The test is easy to perform, takes 10 minutes to provide reliable results and is higly specific to the 2019-nCoV Coronavirus.

NATtrol Zika Virus Stock (Qualitative) (1 mL)

TEST 1 mL
EUR 1106.64
Description: Please contact Gentaur in order to receive the datasheet of the product.

Biocredit Covid-19 Ag Detection Kit

G61RHA20 20 test cards (pkg)
EUR 95

SARS-CoV-2 (COVID-19) Spike Antibody

3525-002mg 0.02 mg
EUR 171.82
Description: Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus (1). The disease is the cause of the 2019–20 coronavirus outbreak (2). The structure of 2019-nCoV consists of the following: a Spike protein (S), hemagglutinin-esterease dimer (HE), a membrane glycoprotein (M), an envelope protein (E) a nucleoclapid protein (N) and RNA. Coronavirus invades cells through Spike (S) glycoproteins, a class I fusion protein. It is the major viral surface protein that coronavirus uses to bind to the human cell surface receptor. It also mediates the fusion of host and viral cell membrane, allowing the virus to enter human cells and begin infection (3). The spike protein is the major target for neutralizing antibodies and vaccine development (4). The protein modeling suggests that there is strong interaction between Spike protein receptor-binding domain and its host receptor angiotensin-converting enzyme 2 (ACE2), which regulate both the cross-species and human-to-human transmissions of COVID-19 (5). The recent study has shown that the SARS-CoV-2 spike protein binds ACE2 with higher affinity than SARS-CoV spike protein (6).

SARS-CoV-2 (COVID-19) Spike Antibody

3525-01mg 0.1 mg
EUR 436.42
Description: Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus (1). The disease is the cause of the 2019–20 coronavirus outbreak (2). The structure of 2019-nCoV consists of the following: a Spike protein (S), hemagglutinin-esterease dimer (HE), a membrane glycoprotein (M), an envelope protein (E) a nucleoclapid protein (N) and RNA. Coronavirus invades cells through Spike (S) glycoproteins, a class I fusion protein. It is the major viral surface protein that coronavirus uses to bind to the human cell surface receptor. It also mediates the fusion of host and viral cell membrane, allowing the virus to enter human cells and begin infection (3). The spike protein is the major target for neutralizing antibodies and vaccine development (4). The protein modeling suggests that there is strong interaction between Spike protein receptor-binding domain and its host receptor angiotensin-converting enzyme 2 (ACE2), which regulate both the cross-species and human-to-human transmissions of COVID-19 (5). The recent study has shown that the SARS-CoV-2 spike protein binds ACE2 with higher affinity than SARS-CoV spike protein (6).

SARS-CoV-2 (COVID-19) Envelope Antibody

3531-002mg 0.02 mg
EUR 171.82
Description: Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus (1). The disease is the cause of the 2019–20 coronavirus outbreak (2). The structure of 2019-nCoV consists of the following: a spike protein (S), hemagglutinin-esterease dimer (HE), a membrane glycoprotein (M), an envelope protein (E) a nucleoclapid protein (N) and RNA. Envelope protein is a small polypeptide that contains at least one alpha-helical transmembrane domain. It involves in several aspects of the virus's life cycle, such as assembly, budding, envelope formation, and pathogenesis. E protein has membrane permeabilizing activity, which provides a possible rationale to inhibit in vitro ion channel activity of some synthetic coronavirus E proteins, and also viral replication (3).

SARS-CoV-2 (COVID-19) Envelope Antibody

3531-01mg 0.1 mg
EUR 436.42
Description: Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus (1). The disease is the cause of the 2019–20 coronavirus outbreak (2). The structure of 2019-nCoV consists of the following: a spike protein (S), hemagglutinin-esterease dimer (HE), a membrane glycoprotein (M), an envelope protein (E) a nucleoclapid protein (N) and RNA. Envelope protein is a small polypeptide that contains at least one alpha-helical transmembrane domain. It involves in several aspects of the virus's life cycle, such as assembly, budding, envelope formation, and pathogenesis. E protein has membrane permeabilizing activity, which provides a possible rationale to inhibit in vitro ion channel activity of some synthetic coronavirus E proteins, and also viral replication (3).

SARS-CoV-2 (COVID-19) NSP7 Peptide

9155P 0.05 mg
EUR 196.25
Description: SARS-CoV-2 (COVID-19) NSP7 Peptide

SARS-CoV-2 (COVID-19) Membrane Peptide

9157P 0.05 mg
EUR 196.25
Description: SARS-CoV-2 (COVID-19) Membrane Peptide

SARS-CoV-2 (COVID-19) NSP8 Peptide

9159P 0.05 mg
EUR 196.25
Description: SARS-CoV-2 (COVID-19) NSP8 Peptide

SARS-CoV-2 (COVID-19) NSP9 Peptide

9161P 0.05 mg
EUR 196.25
Description: SARS-CoV-2 (COVID-19) NSP9 Peptide

SARS-CoV-2 (COVID-19) NSP9 Peptide

9163P 0.05 mg
EUR 196.25
Description: SARS-CoV-2 (COVID-19) NSP9 Peptide

SARS-CoV-2 (COVID-19) Membrane Peptide

9165P 0.05 mg
EUR 196.25
Description: SARS-CoV-2 (COVID-19) Membrane Peptide

SARS-CoV-2 (COVID-19) NSP8 Peptide

9167P 0.05 mg
EUR 196.25
Description: SARS-CoV-2 (COVID-19) NSP8 Peptide

SARS-CoV-2 (COVID-19) Envelope Peptide

9169P 0.05 mg
EUR 196.25
Description: SARS-CoV-2 (COVID-19) Envelope Peptide

SARS-CoV-2 (COVID-19) NSP2 Peptide

9171P 0.05 mg
EUR 196.25
Description: SARS-CoV-2 (COVID-19) NSP2 Peptide

SARS-CoV-2 (COVID-19) NSP2 Peptide

9173P 0.05 mg
EUR 196.25
Description: SARS-CoV-2 (COVID-19) NSP2 Peptide

SARS-CoV-2 (COVID-19) NSP4 Peptide

9175P 0.05 mg
EUR 196.25
Description: SARS-CoV-2 (COVID-19) NSP4 Peptide

SARS-CoV-2 (COVID-19) NSP6 Peptide

9177P 0.05 mg
EUR 196.25
Description: SARS-CoV-2 (COVID-19) NSP6 Peptide

SARS-CoV-2 (COVID-19) NSP10 Peptide

9179P 0.05 mg
EUR 196.25
Description: SARS-CoV-2 (COVID-19) NSP10 Peptide

SARS-CoV-2 (COVID-19) ORF6 Peptide

9189P 0.05 mg
EUR 196.25
Description: SARS-CoV-2 (COVID-19) ORF6 Peptide

SARS-CoV-2 (COVID-19) ORF9b Peptide

9191P 0.05 mg
EUR 196.25
Description: SARS-CoV-2 (COVID-19) ORF9b Peptide

SARS-CoV-2 (COVID-19) ORF3a Peptide

9275P 0.05 mg
EUR 196.25
Description: SARS-CoV-2 (COVID-19) ORF3a Peptide

SARS-CoV-2 (COVID-19) ORF3b Peptide

9277P 0.05 mg
EUR 196.25
Description: SARS-CoV-2 (COVID-19) ORF3b Peptide

SARS-CoV-2 (COVID-19) ORF3b Peptide

9279P 0.05 mg
EUR 196.25
Description: SARS-CoV-2 (COVID-19) ORF3b Peptide

SARS-CoV-2 (COVID-19) ORF3b Peptide

9281P 0.05 mg
EUR 196.25
Description: SARS-CoV-2 (COVID-19) ORF3b Peptide

SARS-CoV-2 (COVID-19) ORF7a Peptide

9283P 0.05 mg
EUR 196.25
Description: SARS-CoV-2 (COVID-19) ORF7a Peptide

SARS-CoV-2 (COVID-19) ORF7a Peptide

9285P 0.05 mg
EUR 196.25
Description: SARS-CoV-2 (COVID-19) ORF7a Peptide

SARS-CoV-2 (COVID-19) ORF8 Peptide

9287P 0.05 mg
EUR 196.25
Description: SARS-CoV-2 (COVID-19) ORF8 Peptide

SARS-CoV-2 (COVID-19) ORF8 Peptide

9289P 0.05 mg
EUR 196.25
Description: SARS-CoV-2 (COVID-19) ORF8 Peptide

SARS-CoV-2 (COVID-19) ORF9c Peptide

9291P 0.05 mg
EUR 196.25
Description: SARS-CoV-2 (COVID-19) ORF9c Peptide

SARS-CoV-2 (COVID-19) ORF10 Peptide

9293P 0.05 mg
EUR 196.25
Description: SARS-CoV-2 (COVID-19) ORF10 Peptide

SARS-CoV-2 (COVID-19) Nucleocapsid Antibody

9099-002mg 0.02 mg
EUR 191.42
Description: Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus. The disease is the cause of the 2019–20 coronavirus outbreak (1). SARS-CoV-2 is the seventh member of the enveloped, positive-stranded RNA viruses that are able to infect humans. The SARS-CoV-2 genome, like other coronaviruses, encodes for multiple structural and nonstructural proteins. The structural proteins include spike protein (S), envelope protein (E), membrane glycoprotein (M), nucleocapsid phosphoprotein (N), and the nonstructural proteins include open reading frame 1ab (ORF1ab), ORF3a, ORF6, ORF7a, ORF8, and ORF10 (2). Nucleocapsid (N) protein is the most abundant protein of coronavirus. It is also one of the major structural proteins and is involved in the transcription and replication of viral RNA, packaging of the encapsidated genome into virions (3), and interference with cell cycle processes of host cells (4). Moreover, in many coronaviruses, including SARS-CoV, the N protein has high immunogenic activity and is abundantly expressed during infection (5). It can be detected in various patient samples including nasopharyngeal aspirate, urine, and fecal. Both S and N proteins may be potential antigens for serodiagnosis of COVID-19, just as many diagnostic methods have been developed for diagnosing SARS based on S and/or N proteins (6).

SARS-CoV-2 (COVID-19) Nucleocapsid Antibody

9099-01mg 0.1 mg
EUR 495.22
Description: Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus. The disease is the cause of the 2019–20 coronavirus outbreak (1). SARS-CoV-2 is the seventh member of the enveloped, positive-stranded RNA viruses that are able to infect humans. The SARS-CoV-2 genome, like other coronaviruses, encodes for multiple structural and nonstructural proteins. The structural proteins include spike protein (S), envelope protein (E), membrane glycoprotein (M), nucleocapsid phosphoprotein (N), and the nonstructural proteins include open reading frame 1ab (ORF1ab), ORF3a, ORF6, ORF7a, ORF8, and ORF10 (2). Nucleocapsid (N) protein is the most abundant protein of coronavirus. It is also one of the major structural proteins and is involved in the transcription and replication of viral RNA, packaging of the encapsidated genome into virions (3), and interference with cell cycle processes of host cells (4). Moreover, in many coronaviruses, including SARS-CoV, the N protein has high immunogenic activity and is abundantly expressed during infection (5). It can be detected in various patient samples including nasopharyngeal aspirate, urine, and fecal. Both S and N proteins may be potential antigens for serodiagnosis of COVID-19, just as many diagnostic methods have been developed for diagnosing SARS based on S and/or N proteins (6).

SARS-CoV-2 (COVID-19) Nucleocapsid Antibody

9103-002mg 0.02 mg
EUR 191.42
Description: Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus. The disease is the cause of the 2019–20 coronavirus outbreak (1). SARS-CoV-2 is the seventh member of the enveloped, positive-stranded RNA viruses that are able to infect humans. The SARS-CoV-2 genome, like other coronaviruses, encodes for multiple structural and nonstructural proteins. The structural proteins include spike protein (S), envelope protein (E), membrane glycoprotein (M), nucleocapsid phosphoprotein (N), and the nonstructural proteins include open reading frame 1ab (ORF1ab), ORF3a, ORF6, ORF7a, ORF8, and ORF10 (2). Nucleocapsid (N) protein is the most abundant protein of coronavirus. It is also one of the major structural proteins and is involved in the transcription and replication of viral RNA, packaging of the encapsidated genome into virions (3), and interference with cell cycle processes of host cells (4). Moreover, in many coronaviruses, including SARS-CoV, the N protein has high immunogenic activity and is abundantly expressed during infection (5). It can be detected in various patient samples including nasopharyngeal aspirate, urine, and fecal. Both S and N proteins may be potential antigens for serodiagnosis of COVID-19, just as many diagnostic methods have been developed for diagnosing SARS based on S and/or N proteins (6).

SARS-CoV-2 (COVID-19) Nucleocapsid Antibody

9103-01mg 0.1 mg
EUR 495.22
Description: Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus. The disease is the cause of the 2019–20 coronavirus outbreak (1). SARS-CoV-2 is the seventh member of the enveloped, positive-stranded RNA viruses that are able to infect humans. The SARS-CoV-2 genome, like other coronaviruses, encodes for multiple structural and nonstructural proteins. The structural proteins include spike protein (S), envelope protein (E), membrane glycoprotein (M), nucleocapsid phosphoprotein (N), and the nonstructural proteins include open reading frame 1ab (ORF1ab), ORF3a, ORF6, ORF7a, ORF8, and ORF10 (2). Nucleocapsid (N) protein is the most abundant protein of coronavirus. It is also one of the major structural proteins and is involved in the transcription and replication of viral RNA, packaging of the encapsidated genome into virions (3), and interference with cell cycle processes of host cells (4). Moreover, in many coronaviruses, including SARS-CoV, the N protein has high immunogenic activity and is abundantly expressed during infection (5). It can be detected in various patient samples including nasopharyngeal aspirate, urine, and fecal. Both S and N proteins may be potential antigens for serodiagnosis of COVID-19, just as many diagnostic methods have been developed for diagnosing SARS based on S and/or N proteins (6).

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